Figure 1
From: Autotetraploid cell Line induced by SP600125 from crucian carp and its developmental potentiality
SP600125-induced polyploidization of crucian carp cells in vitro.
(A) Morphology of the crucian carp cells (passage 0) in vitro, scale bars = 200 μm. (B) Morphology of the crucian carp cells (passage 5) in vitro, scale bars = 200 μm. (C) Karyotype analysis of the crucian carp cells (passage 26), 2N = 100. Scale bars = 20 μm). (D) FACS analysis of SP600125 (blue) or DMSO (red) treated cell DNA content. (E) FACS analysis of DNA content of cells collected from 4n and 8n peaks (cultured for another two passage without SP600125 (blue)), diploid crucian carp cells were used as the control (red).
