Figure 2
From: Autotetraploid cell Line induced by SP600125 from crucian carp and its developmental potentiality
Generation of a stable tetraploid crucian carp cell line.
(A) Scheme for tetraploid crucian carp cells generation by SP600125. (B) FACS analysis of crucian carp cell DNA content after one (blue) or two (green) rounds of DMSO (left) and SP600125 (right) treated, diploid crucian carp cells were used as control. (C) FACS analysis of tetraploid crucian carp cells generated (blue) and diploid crucian carp cell (red) DNA content. (D) Karyotype of tetraploid crucian carp cells (4N = 200), scale bars = 20 μm. (E) FACS analysis of crucian carp cell DNA content after one (blue) or two (green) rounds of Colchicine treated, diploid crucian carp cells were used as control.
