Figure 4
From: Autotetraploid cell Line induced by SP600125 from crucian carp and its developmental potentiality
Jnk pathway did not involve in cell polyploidization.
(A) Western blot analysis detected Jnk (Jnk1/Jnk2) expression in SP600125-induced tetraploid cells and normal diploid cells. Error bars represent the means ± SD; n = 3. P < 0.001. (B) shRNA knockdown of jnk1/jnk3. Cells transfected with an empty vector were used as control. Knockdown was evaluated by Q-PCR. Expression values are relative to actin expression on set as 1. Error bars represent the means ± SD; n = 3. P < 0.001. (C) FACS analysis of jnk1-shRNA (blue) and jnk3-shRNA (green) transfected cell DNA content. An empty vector (red) was transfected as the control.
