Figure 1

Phylogenetic analysis of the core genome of German E. faecium ST192 (a) and non-ST192 (b) clinical isolates. (a) The proportional transformed branch diagram revealed 4 different clades or subclades of ST192 isolates. (b) ST192 and non-ST192 were found to cluster according to their respective MLST type with the exception of UW7027-ST78 falling within clade III of ST192 isolates. Branch labels represent a bootstrap with 1000 permutations. Color assignment was done to visually differentiate ST192 clades as defined in this study (green: VRE clade CIa; red: VRE clade CIb; salmon: VRE clade CII; light blue: VSE/VRE clade CIII and grey VSE/VRE clade IV). Outlying brackets frame the year of isolation of the strains belonging to the respective clade. All isolates are ST192 unless stated next to the strain name. Efm_DO represents the reference E. faecium DO/TX16 (CP003583). “vanB-“ refers to vanB-negative isolates.