Figure 3: Phylogenetic analysis based on the Tn1549 sequences of German E. faecium ST192 and non-ST192 clinical isolates. | Scientific Reports

Figure 3: Phylogenetic analysis based on the Tn1549 sequences of German E. faecium ST192 and non-ST192 clinical isolates.

From: Population structure and acquisition of the vanB resistance determinant in German clinical isolates of Enterococcus faecium ST192

Figure 3

The proportional transformed branch diagram revealed an insertion site-specific clustering across all sequence types. Differentiation of the various insertion sites was further validated by bootstrap analysis with 1000 permutations and is indicated by branch labeling. For consistency, color coding represents the different ST192 clades as represented in Fig. 1a,b (green: VRE clade CIa; red: VRE clade CIb; salmon: VRE clade CII; light blue: VSE/VRE clade CIII and grey VSE/VRE clade IV). Unless indicated by specific ST enumeration, all isolates belonged to ST192. Insertion sites are depicted as locus_tag numbering according to the reference genome E. faecium DO (CP003583). Tn1549 represents the reference sequence used for mapping (CP013009). Insertion site “PAI” represents the reference locus_tag EFAU085_02779, as it is not present in E. faecium DO, and due to the proximity to a pathogenicity island (PAI) was termed “PAI” in the following. unk insertion site unknown.

Back to article page