Figure 4: S1-nuclease macrorestriction of donor, recipient and transconjugant (TC) bacteria.

Separation in PFGE disclosed the plasmid content of the strains analyzed. The red rectangle indicates the putative novel plasmid pWCF-TC1 of 66.5 kb. The red asterisks indicate strains that do not possess the respective plasmid. M marker strain S. aureus NCTC8325.