Figure 1
CuB suppresses cellular migration, invasion and stemness in NSCLC cells at sub-IC50 concentrations.
(A) Wounds were created in subconfluent A549 (Panel A), H1299 (Panel B) and H23 (Panel C) cells and cells were treated with indicated concentrations of CuB for 12, 24 and 48 h. Data were expressed as percent wound closure in treatment groups compared to control. (B) A549, H1299 and H23 cells were seeded in matrigel-coated chambers and treated with indicated concentrations of CuB for 48 h. Data were expressed as percent invasion in treatment groups compared to control (C) A549 and H1299 cells were seeded in ultra-low attachment 96-well plates in 10 replicates with variable concentrations of CuB. The numbers of tumorspheres were counted and represented as average number of tumorspheres per well. (D) The average number of CuB-treated A549 and H1299 colonies containing ≥50 cells were counted and represented as percent of control. Each treatment was repeated in triplicates. Results were obtained from three independent experiments, mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus control.
