Figure 4

CuB inhibits the Wnt/β-catenin signaling in the NSCLC cells.
A549 (Panel A) and H1299 cells (Panel B) were treated with 0–100 nM of CuB for 24 h and whole cell lysates were prepared. Western blot analysis was performed to analyze the expression of Wnt/β-catenin pathway proteins. Blots are representative of three independent experiments. Graphs in (Panel C,D) represent relative band intensities of proteins in A549 and H1299 cells normalized to β-actin. The results were obtained from three independent experiments, mean ± SEM. *p < 0.05 versus Control.