Figure 4 | Scientific Reports

Figure 4

From: Receptor-targeted liposome-peptide-siRNA nanoparticles represent an efficient delivery system for MRTF silencing in conjunctival fibrosis

Figure 4

Silencing efficiency of the liposome-peptide-siRNA nanoparticles with different peptide and liposome formulations.

(A) Using 50 nM siRNA, the MRTF-B gene was efficiently silenced by 76% and 72% with LYR and LER nanoparticles, respectively. LYR and LER nanoparticles showed higher silencing efficiency than PEGylated LYR-P and LER-P nanoparticles. mRNA levels were normalised relative to GAPDH and the results shown are mean ± SD for triplicate experiments; (B) Western blotting showed a significant decrease in the MRTF-B protein expression with LYR and LER nanoparticles; (C) MRTF-B protein silencing was calculated using densitometric analysis and GAPDH as loading control.

Back to article page