Figure 3
From: Foot-and-mouth disease virus non-structural protein 3A inhibits the interferon-β signaling pathway
FMDV 3A interacts with RIG-I, MDA5 and VISA.
(a) FMDV 3A interacts with the overexpression of RIG-I, MDA5 and VISA proteins. The HEK293T cells (2 × 106) were cotransfected with 4 μg of FMDV Flag-3A plasmid and the indicated HA-tagged plasmids (5 μg each). Coimmunoprecipitations were performed with anti-Flag (F) or control IgG (lg) antibodies. Immunoblot analysis was performed with anti-hemagglutinin (HA) (upper panels) antibody. Expression levels of the proteins were analyzed by immunoblot analysis of the lysates with anti-HA and anti-Flag (lower panels). HC: heavy chain; LC: light chain. (b–d) FMDV 3A is associated with endogenous RIG-I, MDA5 and VISA. The HEK293T cells (1 × 107) were cotransfected with 20 μg of FMDV Flag-3A plasmid for 24 hour. Coimmunoprecipitations were then performed with anti-Flag (F), anti-RIG-I, anti-MDA5 or control IgG (lg) antibodies. Immunoblot analysis was performed with anti-VISA, anti-RIG-I or anti-MDA5 (upper panels) antibodies. Expression levels of the proteins were analyzed by immunoblot analysis of the lysates with anti-VISA, anti-RIG-I, anti-MDA5 and anti-Flag-HRP (F-HRP) (lower panels). HA-: HA tag.
