Figure 5
From: Foot-and-mouth disease virus non-structural protein 3A inhibits the interferon-β signaling pathway
Amino acids 1–102 of FMDV 3A inhibits IFN-β signaling pathway by reporter assay.
(a,b) The key domain of FMDV 3A inhibits SeV-induced IFN-β signaling pathway. HEK293T (5 × 104) cells were transfected with the IFN-β reporter or ISRE (100 ng), the indicated expression plasmids (100 ng) and 10 ng of pRL-TK (as an internal control). Twenty hours after transfection, cells were infected with SeV or left uninfected for 12 h before luciferase assays were performed. (c,d) The key domain of FMDV 3A inhibits the IFN-β signaling pathway. HEK293T (5 × 104) cells were transfected with the IFN-β reporter or ISRE (100 ng), the indicated expression plasmids (100 ng) and 10 ng of pRL-TK (as an internal control). Twenty hours after transfection, cells were collected and performed with luciferase assays. Values are presented as the mean ± SD from three independent experiments.
