Figure 4

Chimeric mouse embryos carrying the T (5:7) translocation were developed from blastocysts injected with T (5:7)-GFP-E14 cells.

(a) GFP-positive T (5:7)- mESCs injected into an E3.5 blastocyst. (b,c) Injected GFP-positive T (5:7)-mESCs developed and integrated into the receptive embryo one (b) or two (c) days after injection. (d) One representative E13.5 chimeric embryo developed from blastocyst injected with GFP-positiveT (5:7)-mESCs and contained GFP-positive cells. (e) GFP-positive tissue was isolated from the chest of an E13.5 chimeric embryo. (f) Cultured cells isolated from the hind legs of the chimeric embryos. Some GFP-positive cells shown the contribution of T (5:7)-GFP-E14 cells into the chimeric embryos. Scale bars represent 100 μm. (g,h) Both the T (5:7) chromosome-long and the T (5:7) chromosome-short were detected in chimeric mouse embryos by PCR and sequencing analysis.