Figure 3: Phenotypic analyses of the metabolism of sterol by M. neoaurum ATCC25795 and its derivative strains.
From: Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism
(a) HPLC chromatogram comparison of the products from the transformation of 2 g/l of cholesterol in MYC/02 media at 30 °C by strains M. neoaurum ATCC 25795 (blue), NwIB-XII (red) and XIIp261hsd4A (green). Cholesterol can be completely degraded by M. neoaurum ATCC 25795 without obvious accumulation of intermediates. The catabolism of cholesterol in NwIB-XII was blocked to accumulate multiple metabolites, including ADD (I), AD (II), TS (III) and BD (IV). The plasmid pMV261-hsd4A was electro-introduced into NwIB-XII, resulting in the XIIp261hsd4A strain. Strain XIIp261hsd4A transformed cholesterol to ADD (I) and AD (II). (b) Conversion relationship between the metabolites (I to IV). Arrows in purple signify the function of Hsd4AMN deduced from the product phenotypes of strains NwIB-XII and XIIp261hsd4A. Hsd4AMN can irreversibly catalyze the oxidation of TS to AD and BD to ADD, respectively. AD, androst-4-ene-3,17-dione; ADD, androst-1,4-dien-3,17-dione; BD, boldenone; TS, testosterone.
