Table 1 Summary statistics of the sequencing and the de novo transcriptome assembly of the Potamotrygon amandae and P. falkneri cDNA samples.
P. amandae | P. falkneri | |
---|---|---|
Before pre-processing | ||
Number of raw reads | 14,539,142 | 15,375,430 |
Raw data (bp) | 3,357,743,092 | 3,384,969,885 |
Average read length (bp) | 231 | 220 |
After pre-processing | ||
Number of high-quality reads | 13,109,527 | 13,730,400 |
Clean data (bp) | 2,637,602,285 | 2,627,406,006 |
% of reads with Phred score ≥ 33 | 92.7 | 92.4 |
Average read length (bp) | 200 | 207 |
Trinity assembly statistics | ||
Number of contigs | 147,881 | 105,191 |
Number of contigs ≥ 1FPKM | 75,083 | 49,219 |
Number of contigs ≥ 1 FPKM and containing CDSs | 25,092 | 22,083 |
Contigs (bp) | 50,987,297 | 42,504,708 |
N50 | 3,015 | 2,700 |
Average contig length (bp) | 2,032 | 1,924 |
Min. contig length (bp) | 293 | 297 |
Max. contig length (bp) | 15,915 | 14,247 |