Table 1 Histopathological analysis of renal injury in Brown Norway versus Sprague Dawley rats.

From: Macrophage-derived Lipocalin-2 contributes to ischemic resistance mechanisms by protecting from renal injury

Experimental group

Epithelial necrosis

Epithelial balloonization

Tubular dilatation

Detachment

Oedema

Total score

Brown Norway rats

 Sham

0.3+/−0.1

0.4+/−0.01

0

0.2+/−0.03

0

0.9+/−0.14

 I/R_24 h

2.0+/−0.1

1.3+/−0.04

0.8+/−0.1

1.9+/−0.4

0.1+/−0.03

6.1+/−0.67*

 I/R_MACS

1.2+/−0.4

0.9+/−0.08

0.4+/−0.05

0.9+/−0.06

0.6+/−0.06

4.0+/−0.65

 I/R_siMACS

2.4+/−0.2

1.5+/−0.08

1.0+/−0.05

2.5+/−0.05

0.6+/−0.06

8.4+/−0.44+

 I/R_scMACS

1.3+/−0.3

0.8+/−0.1

0.5+/−0.04

0.8+/−0.04

0.6+/−0.06

4.0+/−0.54

Sprague Dawley rats

 Sham

0.6+/−0.03

0.4+/−0.03

0.2+/−0.01

0.5+/−0.03

0

1.7+/−0.1

 I/R_24 h

2.9+/−0.1

1.5+/−0.05

1.9+/−0.02

2.5+/−0.2

0.4+/−0.03

9.2+/−0.6

 I/R_MACS

0.8+/−0.6

0.4+/−0.08

0.7+/−0.05

1.9+/−0.06

0.2+/−0.06

4.0+/−0.06

 I/R_Lcn-2

0.7+/−0.6

0.3+/−0.08

0

0.3+/−0.06+

0

1.3+/−0.74§

 I/R_β-gal

1.0+/−0.06

0.6+/−0.08

0.8+/−0.02

1.3+/−0.06

0.4+/−0.05

4.1+/−0.27

  1. Histological analysis indicates that BN rats, as compared to SD rats, preserve significantly higher tissue integrity after ischemia/reperfusion injury. Less cellular infiltration, tubular cell balloonization and severe necrosis could be observed. Transfer of Lcn-2 knockdown macrophages to BN animals promoted renal injury, whereas transfer of Lcn-2 over-expressing macrophages to SD rats significantly enhanced protection against IRI. Data are represented as means ± S.E.M. n = 5; *p < 0.05 BN I/R_24 h vs. SD I/R_24 h, +p < 0.05 BN I/R_siMACS vs. BN I/R_scMACS, §p < 0.05 SD I/R_Lcn-2 vs. SD I/R_β-gal.
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