Table 2 Analysis of renal regeneration markers in Brown Norway versus Sprague Dawley rats.

From: Macrophage-derived Lipocalin-2 contributes to ischemic resistance mechanisms by protecting from renal injury

Experimental group

PCNA-positive

Stathmin positive

Brown Norway rats

 Sham

1+/−0.5

2+/−0.2

 I/R_24 h

7+/−0.6*

9+/−0.8*

 I/R_MACS

6+/−0.9

8+/−0.7

 I/R_siMACS

3+/−0.5+

2+/−0.1+

 I/R_scMACS

8+/−0.9

7+/−0.7

Sprague Dawley rats

 Sham

1+/−0.3

1+/−0.1

 I/R_24 h

4+/−0.2

5+/−0.2

 I/R_MACS

8+/−0.4

7+/−0.5

 I/R_Lcn-2

18+/−1.2§

15+/−1.5§

 I/R_β-gal

7+/−0.9

5+/−0.6

  1. The numbers depict averaged PCNA- and stathmin-positive cell counts per field. 5 fields were counted per section. Results indicated that BN rats, as compared to SD rats, represent significantly higher renal cell proliferation after ischemia/reperfusion injury. Transfer of Lcn-2 knockdown macrophages to BN animals induced a loss of regeneration markers at 24 h of reperfusion, whereas transfer of Lcn-2 over-expressing macrophages to SD rats significantly promoted renal regeneration. Data are represented as means ± S.E.M. n = 5; *p < 0.05 BN I/R_24 h vs. SD I/R_24 h, +p < 0.05 BN I/R_siMACS vs. BN I/R_scMACS, §p < 0.05 SD I/R_Lcn-2 vs. SD I/R_β-gal.
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