Figure 1: Expression and purification of the recombinant EV71, CA16, CA5, CA6, CB3, PV1 and HAV VP1 proteins. | Scientific Reports

Figure 1: Expression and purification of the recombinant EV71, CA16, CA5, CA6, CB3, PV1 and HAV VP1 proteins.

From: Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals

Figure 1

(a) Purified recombinant VP1 proteins were separated on 12% SDS-PAGE gel and stained with Coomassie blue. The expression of the recombinant EV71, CA16, CA5, CA6, CB3, PV1 and HAV VP1 proteins was induced with IPTG. The relative molecular weights (MW) of the expressed products were 50.37 kDa for EV71, 50.38 kDa for CA16, 50.20 kDa for CA5, 50.86 kDa for CA6, 50.45 kDa for CB3, 51.16 kDa for PV1 and 48.08 kDa for HAV. The expressed products were purified using Ni-NTA column affinity chromatography. (b) Western blot analysis of the expression of His-probe recombinant EV71, CA16, CA5, CA6, CB3, PV1 and HAV VP1 proteins.

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