Figure 1
PC potentiates virus-triggered interferon induction.
(A) A549 cells were co-transfected with IFN-β, IFN-λ1, NF-κB and ISRE luciferase reporter constructs and control vector or PC expression plasmids. pRL-TK was cotransfected as an internal control in the luciferase assay. Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. *p < 0.05, **p < 0.01. no sign., no significant difference (one-way ANOVA). (B) A549 cells were transfected with vector or PC expression plasmids for 24 h and then infected with SeV (MOI = 1) for 6 h. IFN mRNA levels were measured by qPCR. *p < 0.05, **p < 0.01 (one-way ANOVA). (C) A549 cells were transfected with the vector or PC expression plasmids for 24 h and then infected with SeV (MOI = 1) for 12 h. PKR and Mx1 mRNA levels were determined by qPCR. PKR, Mx1 and PC proteins were assessed by western blot, respectively. *p < 0.05 (one-way ANOVA). All graphs represent means standard deviations for 3 experiments.
