Figure 3
Identification of PC as a positive regulator of virus-induced pro-inflammatory cytokines.
(A) A549 cells were transfected with vector or PC expression plasmids for 24 h and then infected with SeV (MOI = 1) for 6 h. IL-6, IL-8, IL-1β and TNFα mRNA levels were measured by qPCR. (B) The same numbers of PC-KD and Scr.-KD cells were infected with SeV (MOI = 1) for 6 h. IL-6, IL-8, IL-1β and TNFα mRNA levels were measured by qPCR. (C) PC-KD and Scr.-KD cells were transfected with vector or PC expression plasmids for 24 h and then infected with SeV (MOI = 1) for 6 h. IL-6, IL-8, IL-1β and TNFα mRNA levels were measured by qPCR. (D) A549 cells were co-transfected with IL-6 or IL-8 luciferase reporter plasmids and vector or PC expression plasmids. pRL-TK was cotransfected as an internal control in the luciferase assay. Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. **p < 0.01 (one-way ANOVA). All graphs represent means standard deviations for 3 experiments.
