Figure 4
PC positively regulates cellular antiviral response.
A549 cells were transfected with vector or PC expression plasmids for 24 h were infected with IAV (MOI = 1) for another 24 h and cells were harvested for total RNA isolation. Relative levels of NP-specific mRNA, cRNA and vRNA were measured by qPCR. **p < 0.01 (one-way ANOVA). (B) A549 cells were transfected with PC-specific siRNA or nonsense control siRNA for 24 h. Cells were harvested 24 h after infection with IAV (MOI = 1). Relative levels of NP-specific mRNA, cRNA and vRNA were measured by qPCR. **p < 0.01 (one-way ANOVA). (C) PC-KD and Scr.-KD cells were transfected with vector or PC expression plasmids for 24 h. The cells were harvested 24 h after IAV infection (MOI = 1) and the relative levels of NP-specific mRNA, cRNA and vRNA were measured by qPCR. **p < 0.01 (one-way ANOVA). (D) RD cells were transfected with vector or PC expression plasmids for 24 h. Twelve hours after EV71 infection (MOI = 1), VP1 protein levels were determined by western blot. (E) A549 cells were transfected with vector or PC expression plasmids for 24 h and then infected with VSV-eGFP (MOI = 1) for 6 h. VSV-eGFP replication was visualized by fluorescence microscopy (right panel). Twenty-four hours post-infection, the supernatants were harvested and analyzed for VSV production using a standard plaque assay (left panel). **p < 0.01. (F) Vero cells were transfected with vector or PC expression plasmids for 24 h and then infected with VSV (MOI = 1). VSV production in the supernatants was estimated as in (E) no sign., no significant difference. All graphs represent means standard deviations for 3 experiments.
