Figure 5
PC regulates virus-triggered signaling through MAVS.
(A) A549 cells were transfected with MAVS-specific siRNA or nonsense control siRNA for 48 h and MAVS mRNA and protein levels were determined by qPCR and western blotting, respectively. *p < 0.05 (one-way ANOVA). (B) A549 cells were co-transfected with vector or PC expression plasmids and the indicated MAVS-specific siRNA or nonsense control siRNA together with IFN-β and IFN-λ1 luciferase reporter plasmids. pRL-TK was cotransfected as an internal control in the luciferase assay. Twenty-four hours after transfection, cells were infected with SeV (MOI = 1) for 12 h before reporter assays were performed. *p < 0.05, **p < 0.01 (one-way ANOVA). HEK293T cells were co-transfected with HA-PC and Flag-MAVS expression plasmids (C), HA-TRAF6 and Flag-PC or Flag-TRAF6 and HA-PC expression plasmids (D), HA-PC and Flag-TRADD expression plasmids (E), HA-TRAF3 and Flag-PC expression plasmids (F), or HA-PC and Flag-NEMO expression plasmids (G) for 48 h. Co-immunoprecipitation and immunoblot analyses were performed with the indicated antibodies. All experiments were repeated at least three times with consistent results.
