Figure 4
The effect of akermanite extract on the expression of osteogenic, angiogenic and osteoclastogenic genes and the activation of signaling pathways.
(A) Rea-time PCR analysis of Runx2 (a), BMP-2 (b), BSP (c), OPN (d), OCN (e), OPG (f), RANKL (g), TNF-α (h), VEGF (i) and ANG-1 (j) in BMSCs-OVX treated with β-TCP and akermanite extracts at days 4 and 7. The cells cultured without β-TCP and akermanite extracts was treated as control group (Control). ★indicates significant differences as compared with control group; ▲indicates significant differences as compared with β-TCP group, p < 0.05; (B) Western blot assay for key protein expression of MAPK, AKT and STAT3 signaling pathways for BMSCs-OVX treated with β-TCP and akermanite extracts at 30, 60 and 90 min; (C) The quantitative assay for the ratios of p-P38/P38 (a), p-ERK/ERK (b), p-JNK/JNK (c), p-AKT/AKT (d) and p-STAT3/STAT3 (e). ★indicates significant differences as compared with β-TCP group, p < 0.05.
