Figure 6
In vitro osteoclastogenesis assay.
(A) TRAP staining for BMMs cultured in M-CSF and RANKL supplement with β-TCP and akermanite extracts, respectively; (B,C) The quantitative assay for number (B) and area (C) of osteoclasts according to TRAP staining; (D–F) Rea-time PCR analysis of TRAP (D), cathepsin K (E) and NFATc1 (F) for BMMs cultured in M-CSF and RANKL supplement with β-TCP and akermanite extracts, respectively. The cells cultured in M-CSF and RANKL without β-TCP and akermanite extracts was treated as control group (Control). ★indicates significant differences as compared with control group; ▲indicates significant differences as compared with β-TCP group, p < 0.05. Scale bar = 100 μm.
