Figure 4 | Scientific Reports

Figure 4

From: Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment

Figure 4

Effects of Pygo2 knockdown on the activity of the canonical Wnt signal pathway in glioma cell lines.

(A,B) Effects of Pygo2 knockdown by shRNAs on TOP flash luciferase activity in human brain glioma U-87MG and U251 cell lines. Pygo2 shRNA1-3 and control (scr-shRNA) were used. (C,D) Efficiency of Pygo2 knockdown by shRNAs. (E–H) Effects of Pygo2 knockdown on Wnt/β-catenin pathway component genes in human brain glioma U-87MG and U251 cell lines. Western blot analysis was used to check the expression of the target genes in U-87MG and U251 cell lines, with β-actin used as a control. Arrows (→) indicate cropping lines and gels were obtained under the same experimental conditions. (E,F) QRT-PCR analysis used to determine the mRNA expression (G,H). **P < 0.01 and ***P < 0.001.

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