Figure 5 | Scientific Reports

Figure 5

From: Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment

Figure 5

Effects of Pygo2 knockdown on H3K4me2 and -3 protein expression.

(A–D) Effects of Pygo2 knockdown by shRNAs on the expressed of H3K4me2 and -3, present on the promoters of CD44 and CyclinD1, in human brain glioma U-87MG (A) and U251 (B) cell lines. The enrichment of H3K4me2/3 was measured by CHIP. Histone H3 was used as a negative control. (E,F) Effects of Pygo2 knockdown by shRNAs on the total protein expression of H3K4me2 and -3 in human brain glioma U-87MG (E) and U251 (F) cell lines. The protein levels were measured by western blot assay, with histone H3 used as a negative control. Arrows (→) indicate cropping lines and gels were obtained under the same experimental conditions. **P < 0.01 and ***P < 0.001.

Back to article page