Table 3 Primers for Pygo2 shRNAs, qRT-PCR and CHIP.
Name | Sequence | Length | |
|---|---|---|---|
Pygo2 shRNAs | |||
Pygo2 shRNA1 | F | 5′-CACCGCCGGTCTGCAAATGAAGAGTTCAAGAGACTCTTCATTTGCAGACCGGC-3′ | 53 bp |
R | 5′-AAAAGCCGGTCTGCAAATGAAGAGTCTCTTGAACTCTTCATTTGCAGACCGGC-3′ | 53 bp | |
Pygo2 shRNA2 | F | 5′-CACCGAAGCGAAGGAAGTCAAATACTCAAGAGGTATTTGACTTCCTTCGCTTC-3′ | 53 bp |
R | 5′-AAAAGAAGCGAAGGAAGTCAAATACCTCTTGAGTATTTGACTTCCTTCGCTTC-3′ | 53 bp | |
Pygo2 shRNA3 | F | 5′-CACCAGGCCGGTCTGCAAATGAAGATCAAGAGTCTTCATTTGCAGACCGGCC-3′ | 52 bp |
R | 5′-AAAAGGCCGGTCTGCAAATGAAGACTCTTGATCTTCATTTGCAGACCGGCCT-3′ | 52 bp | |
qRT-PCR | |||
Pygo2 | F | 5′-AGAAAAGAAGCGAAGGAAGTCAAA-3′ | 24 bp |
R | 5′-GGTGATCCACCATGGGAGTT-3′ | 20 bp | |
P | 5′ FAM-CTCACATCTGACGGAGTTTGCACCACC-BHQ 3′ | 27 bp | |
CyclinD1 | F | 5′- GCGTGTAGCTATGGAAGTTGCA-3′ | 22 bp |
R | 5′- CATCCCGAATGAGAGTCCTACAG-3′ | 23 bp | |
P | 5′ FAM-CTTACGTGCCACCACGGCGTTGT-BHQ 3′ | 23 bp | |
C-myc | F | 5′-ATCTCACAGTGACCAACCCAAA-3′ | 22 bp |
R | 5′- TCGGTCACGGAGCCAATC-3′ | 18 bp | |
P | 5′ FAM-CGTGAACGAGAAGTCCTGCAACTGCC-BHQ 3′ | 26 bp | |
CyclinA | F | 5′- ATGAAGAAACAGCCAGACATCAC-3′ | 23 bp |
R | 5′- CTCTCAGCACTGACATGGAAG-3′ | 21 bp | |
P | 5′- TGAGAGCTATCCTCGTGGACTGGTTAGTTGA-BHQ 3′ | 31 bp | |
CD44 | F | 5′- AGTCGTCAGAAACTCCAGACCAGT-3′ | 24 bp |
R | 5′- CCAGCTCCCTGTAATGGTTATGTT-3′ | 24 bp | |
P | 5′- CAGCTGATGAGACAAGGAACCTGCAGAA-BHQ 3′ | 28 bp | |
CHIP | |||
CyclinD1 promoter | F | 5′-CACCCCCAACAAAACCAATTAG-3′ | 22 bp |
R | 5′-ACGTTACTGTTGTTAAGCAAAGATCA-3′ | 26 bp | |
CD44 promoter | F | 5′- CAAACACACACATTTTGCAGCATAT-3′ | 25 bp |
R | 5′- CCCTCTGGTACGGAATAAAACTCA-3′ | 24 bp | |
