Figure 1: KAR positively regulates the transcription of marker genes during the soybean seed germination process.

The expression of KAR-inducible genes (GmSTH7 and GmGUF1) was investigated by qRT-PCR during the imbibition process. Dry seeds and imbibed seeds (3, 6, 9, 12, 24 hours after imbibition) were used for total mRNA extraction. Three replications were performed and similar results were obtained. Primer pairs of the marker genes are listed in Supplementary Table 1.