Figure 5: Gene expression analysis during the course of imbibition.

Gene expression was investigated by qRT-PCR during the course of imbibition. Dry seeds and imbibed seeds (3, 6, 9, 12, 24 hours after imbibition) were used for total RNA isolation. Three biological replications were performed. (a) ABA biosynthesis gene, GmAAO; (b) positive regulators genes of ABA signaling pathway; and (c) GA biosynthesis genes.