Figure 4: PS341 induces cell cycle arrest in HCC and CRC cells.
From: PS341 inhibits hepatocellular and colorectal cancer cells through the FOXO3/CTNNB1 signaling pathway
(a) PS341 induces G0/G1 cell cycle arrest in both HCC and CRC cells. DMSO or PS341 were supplemented into the medium of HepG2, Huh7, HT29 and LoVo cells and cell cycle analysis was processed after 70% ethanol fixation and PI staining. (b) The RT-PCR analysis of the mRNA expression levels of Cyclin A2, Cyclin B1, Cyclin D1, CDK1, CDK4 and E2F1 in HCC and CRC cells with PS341 treatment. (c) PS341 inhibits the expression of Cyclin D1 and CDK4 at transcriptional level in HCC and CRC cells. (d) PS341 suppresses the expression of Cyclin D1 and CDK4 at the protein level in HCC and CRC cells. The whole assay was repeated three times. The full-length blots/gels are presented in Supplementary Figs 3 and 4. Data are displayed as mean ± SD. *P < 0.05, **P < 0.01.
