Table 2 Biological evaluation of haliangicin (1) and its analogues (4 and 7) obtained through gene disruption.

From: Heterologous Production of the Marine Myxobacterial Antibiotic Haliangicin and Its Unnatural Analogues Generated by Engineering of the Biochemical Pathway

Organisms

1

4

7

PC

Phytophthora capsici (μg disk–1)

0.1

0.1

3.0

0.1

Candida rugosa (MIC, μg mL–1)

>32

>32

>32

0.13

Bacillus subtilis (MIC, μg mL–1)

>32

>32

>32

0.08

Escherichia coli (MIC, μg mL–1)

>32

>32

>32

8.0

HeLa S3 cells (IC50, nM)

41

55

17

8.6

  1. Growth inhibition of the oomycete P. capsici was evaluated by a disk diffusion test and denoted as minimum doses to form a definite inhibition zone. Metalaxyl was used as a positive control (PC). In MIC test, PCs were amphotericin B for C. rugosa and ampicillin for B. subtilis and E. coli. The cytotoxicity against HeLa cells was determined by MTT test and paclitaxel was used as PC.
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