Figure 1: Detection of propionyl-CoA assay product by LC-MS. | Scientific Reports

Figure 1: Detection of propionyl-CoA assay product by LC-MS.

From: Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans

Figure 1

Main Figure, Extracted ion chromatogram at 824 m/z. Top, the Cphy1178(20–462) assay mixture containing (50 nM Cphy1178(20–462), 75 μM NAD+, 100 μM CoA, 10 mM propionaldehyde, 100 mM Tris.HCl pH 8.0, 100 mM KCl); middle, 10 ug propionyl-CoA control; bottom, negative control. Insert, mass spectrum of the Propionyl-CoA obtained by summing the spectra between 4.8 to 5.8 minutes. Top, Cphy1178(20–462) assay; bottom, propionyl-CoA control. (predicted monoisotopic mass [M+H]+ C24H41N7O17P3S; 824.1487 Da).

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