Figure 2 | Scientific Reports

Figure 2

From: Neuroprotective Effects of A Standardized Flavonoid Extract of Safflower Against Neurotoxin-Induced Cellular and Animal Models of Parkinson’s Disease

Figure 2

(a) High-content images of fluorescently-labeled β-tubulin III in differentiated PC12 cells pretreated with K3R or AYB after rotenone-induced damage. Microtubule structure (green) was visualized by fluorescent labeling using anti-β-tubulin III. Nuclei were counterstained using Hoescht33342 (blue). (b) Effects of K3R and AYB on the proportion of positive cells after rotenone-induced damage. (c) Effects of K3R and AYB on the fluorescent intensity for β-tubulin III in the positive cells after rotenone-induced damage. (d) Effects of K3R and AYB on the cell area after rotenone-induced damage. HCA was analyzed with the Columbus™ software. The cells that were greater than 38 μm in length and had neurite were defined as the positive cells (solid arrows). Cells were pretreated with K3R or AYB (50, 100 and 200 μM) for 6 h before rotenone treatment for 24 h. Bar = 100 μm. Data are the mean ± SEM, n = 9, ##P < 0.01 vs. untreated control (CTR) group; *P < 0.05, **P < 0.01 vs. rotenone group.

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