Figure 2 | Scientific Reports

Figure 2

From: Essential Roles of RNA-binding Protein HuR in Activation of Hepatic Stellate Cells Induced by Transforming Growth Factor-β1

Figure 2

HuR closely correlates with fibrosis markers and binds to SphK1 mRNA in mouse fibrotic livers.

The correlation between mRNA levels of HuR and α-SMA (a), Col α1(I) (b), TGF-β1 (c) or SphK1 (d) in mouse fibrotic livers. (e) Liver non-parenchymal cells of Sham or BDL mice were isolated and RIP analysis was performed. SphK1 mRNA was measured by qRT-PCR and the PCR products were size-fractionated in a 2% agarose gel. The gels are run under the same experimental conditions. (f) Liver non-parenchymal cells of mice treated with OO or CCl4 were isolated and RIP analysis was performed as described.

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