Figure 2: Immunocytochemical and immunocytofluorescent staining, WB of MT1-MMP protein expression in transfected cells. | Scientific Reports

Figure 2: Immunocytochemical and immunocytofluorescent staining, WB of MT1-MMP protein expression in transfected cells.

From: Membrane type 1-matrix metalloproteinase induces epithelial-to-mesenchymal transition in esophageal squamous cell carcinoma: Observations from clinical and in vitro analyses

Figure 2

(Panel a) Undetectable expression of MT1-MMP protein in ESCC cell lines transfected with pcDNA3.1 vector (Mock; 400×). (Panel b) Intense cytoplasmic and membranous staining of MT1-MMP in ESCC cell lines transfected with MT1-MMP plasmids (400×). (Panel c) Immunofluorescence image showing the absence of green fluorescence in the cytoplasm and plasma membrane of cell lines transfected with the empty vector (mock; 400×). (Panel d) Immunofluorescence image showing green fluorescence in the cytoplasm and plasma membrane of ESCC cell lines transfected with MT1-MMP plasmids (400×). (Panel e) Expression of the 42-kDa β-actin protein in cells transfected with the empty vector (mock) and ESCC cell lines transfected with MT1-MMP plasmids by western blotting. The 66-kDa MT1-MMP protein was detected in ESCC cell lines transfected with the MT1-MMP plasmids, but was undetectable in those transfected with mock.

Back to article page