Figure 2 | Scientific Reports

Figure 2

From: Differential expression of p38 MAPK α, β, γ, δ isoforms in nucleus pulposus modulates macrophage polarization in intervertebral disc degeneration

Figure 2

Immunofluorescence staining of NP tissue with antibodies against cell Immunophenotype.

(A) cells in NP were immunolabelled for CD68 (green) and CD45 (red), cell nucleus were blue counterstained using DAPI. (B) In degenerated NP, almost all NP cells (CD24, red) were co-stained with CD68 (green). (C) all cells express CD68 positive, but CD11b immunopositivity were only present in a few cells. (D,E) cells in degenerated NPs, showed either CD24 positive or CD11 positive. (F) percentage of cells positive for respective markers in the NP of IDD (Grade III, IV, V) compared with control (Grade II) (In these experiments, all 300 human samples were used, *P < 0.05.) Bar = 50 μm.

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