Figure 5 | Scientific Reports

Figure 5

From: Differential expression of p38 MAPK α, β, γ, δ isoforms in nucleus pulposus modulates macrophage polarization in intervertebral disc degeneration

Figure 5

Real-time RT-PCR analysis of cells transfected with a lentivirus expressing control shRNA (sh-C), sh-p38α, sh-p38β, sh-p38γ or sh-p38δ after IL-1β treatment for 24h.

(A,B) mRNA and protein expression of p38α, p38β, p38γ or p38δ in cells transfected with a lentivirus expressing control shRNA (sh-C), sh-p38α, sh-p38β, sh-p38γ or sh-p38δ (n = 3, respectively). (C–F) the mRNA expression of ADAMTS-4,-5, MMP-13 and CCL3 were analyzed (n = 3, respectively). (G,H) the mRNA expression of collagen II and aggrecan were analyzed (n = 3, respectively). Data show the mean ± SEM from 3 independent experiments. *P < 0.05.

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