Figure 3
From: Resistin Induces Hypertension and Insulin Resistance in Mice via a TLR4-Dependent Pathway
mRNA and plasma levels of BP-regulatory genes.
mRNA and plasma levels of BP-regulatory genes were measured in WT mice and tlr4−/− mice. (A) Angiotensinogen (Agt) was detected in the liver; (B) plasma ANG II levels; (C) ACE, (D) eNOS, (E) ETA and (F) ETB were detected in the lung; (G) Inhibition efficiency of tlr4 by siRNA, NC group was transfected with scrambled siRNA; (H) Inhibiting tlr4 blocked the stimulation effect of resistin on Agt expression, NC and resistin groups were transfected with scrambled siRNA; (I) eNOS and ET-1 were measured in EA.hy 926 cells. Data are presented as mean ± sd. (for studies in vivo, n = 3–6; for studies in vitro, 3 independent experiments are performed) **P < 0.01, ***P < 0.001.
