Figure 5
MiR-133α/AFTPH axis regulated expression of proteins related to endosome and trans-Golgi network trafficking pathways.
(A) NCM460-NTR1 cells transfected with as-miR-133α and NCM460-NTR1-AFTPH cells showed increased AFTPH expression in In-cell ELISA assay, when compared to their respective controls. (B) Rab5 expression was increased in NCM460-NTR1-AFTPH cells, when compared to NCM460-NTR1 cells. (C) Rab7 expression was increased in miR-133α gene-silenced NCM460-NTR1 cells when compared to the controls. (D) Rab11 expression was increased in both NCM460 cells transfected with as-miR-133α and NCM460-NTR1-AFTPH cells when compared to their respective controls. (E) Increase in TGN38 expression was observed in NCM460-NTR1-AFTPH cells when compared with controls. (F) Golgin97 expression was increased in NCM460-NTR1 cells transfected with as-miR-133α, when compared with controls. *p < 0.05, **p < 0.01, ***p < 0.005 when compared with respective controls.
