Figure 7
A schematic diagram showing the proposed mechanism for intracellular NTR1 trafficking regulated by NT-mediated miR-133α/AFTPH axis in human colonic epithelial cells.
NT/NTR1 coupling induced detachment of negative transcription regulator ZEB1 from the promoter region of miR-133α in nucleus in human colonic epithelial cells. Increased miR-133α transcription reduced endogenous AFTPH levels in TGN in vitro and promoted intracellular NTR1 trafficking to cell membrane. Our results suggested failure to reduce endogenous AFTPH levels after NT stimulation caused NTR1 retention in cytoplasm, which coincided with increased Rab11 expression in endosome trafficking pathway.
