Figure 1: Decreased Bone Mass in long bone from Mysm1−/− Mice

. (a) qRT-PCR of mRNA from tibia tissues of Mysm1−/− mice and WT mice, n = 5. (b) Micro CT analysis of the sectioned longitudinal sections and cross sections of the distal ends of tibias, n = 7. (c) Quantification of trabecular bone fraction. Bone volume/ total volume (BV/TV), bone surface/total volume(BS/TV), trabecular bone number(Tb.N), trabecular thickness (Tb. Th), bone surface/bone volume(BS/BV), and trabecular bone space(Tb.Sp), 6-week and 10-week old mice. n = 4. (d) H&E staining of longitudinal sections through the tibia at the metaphysis showing the decreased amount of bone trabeculae (arrows) present in 3-week, 6-week and 10-week old Mysm1−/− mice. n = 3. (e) Fluorescent micrographs of the double labeled mineralization fronts at the mid-diaphysis of the tibias of 3-week-old Mysm1−/− and WT littermate mice. The brackets between the two labeling, tetracyclin at the top and calcein at the bottom indicate the amount of newly formed bone. Scale bar, 200 μm and 50 μm, respectively. The right panel is the bone formation rates (BFRs) of 3-week-old Mysm1−/− and WT littermate mice, n = 4. (f) Longitudinal sections through the tibia at the metaphysis stained with toluidine blue of 6-week-old Mysm1−/− and WT littermate mice; showing the decreased amount of bone trabeculae (arrows) present in the Mysm1−/− mice. Scale bar, 200 μm and 80 μm, respectively. Right panel is the osteoid thickness of 6-week-old Mysm1−/− and WT littermate mice, n = 5. (g) Longitudinal sections through the tibia at the metaphysis stained with golden staining of 10-week-old Mysm1−/− and WT littermate mice; showing the decreased amount of osteoblasts (stained with yellow, yellow arrow) and the amount of the osteoid (stained with red, black arrow) present in the Mysm1−/− and WT littermate mice. Scale bar, 200 μm and 80 μm, respectively. Right panel is the osteoblasts number of 10-week-old Mysm1−/− and WT littermate mice, n = 6. Error bar is ± SEM; *p < 0.05; **p < 0.01.