Figure 2: Decreased Bone Mass in calvaria from Mysm1−/− Mice.

(a) Coronal sections of the calvaria stained with toluidine blue and double calcein labeling of 2 week old Mysm1−/− mice and WT littermate; Scale bar, 200 μm n = 5. (b) qRT-PCR to measure mRNA from calvaria tissues, n = 5. (c) The amount of bone Osteoid present in calvaria was increased in 3-week old Mysm1−/− mice, compared with WT littermate mice. n = 5. (d) The calvaria bi-parietal diameter of Mysm1−/− mice is much shorter than that of WT littermate mice, n = 5. (e) The bone formation rates (BFRs) of 3-week old Mysm1−/− mice is much lower than those of WT littermate mice, n = 5. (f) qRT-PCR to measure Alp, Runx2, Ocn, Ppar-γ, Adi mRNA levels in calvaria tissues from 3-week and 10-week old Mysm1−/− mice, compared with WT littermate mice (set as 1), n = 5. Error bar is ± SEM; *p < 0.05; **p < 0.01.