Figure 1 | Scientific Reports

Figure 1

From: Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Figure 1

Schematic workflow for identifying microbial enzyme-encoding genes by activity-based single-cell sequencing using microdroplets.

(a) The workflow includes single microbial cell isolation in W/O microdroplets (step 1), activity-based single-cell screening and recovery of target cells (step 2), whole genome amplification (step 3) and genome sequencing (step 4). Genes encoding the target enzymes are identified based on genomic information (step 5). The entire process could be completed in 4–5 days. (b) Schematic representation of single microbial cell isolation in W/O microdroplets using a microfluidic device. (c) Schematic representation of activity-based single-cell screening and recovery of target cells.

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