Figure 2 | Scientific Reports

Figure 2

From: Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Figure 2

Isolation and genome amplification of bacteria exhibiting BGL activities from surface and deep seawater.

(a) Bright-field (left) and fluorescence (right) images of W/O microdroplets encapsulating environmental bacteria with FDGlu. The white arrowhead shows a fluorescent bacterial cell in a W/O microdroplet. Scale bar represents 20 μm. (b) PCR amplification of 16S rRNA genes from MDA products. The amplicons were analysed with 1% agarose gel electrophoresis and stained with SYBR Safe. The estimated amplicon size is approximately 1,466 bp. Lane M, DNA marker (λ-EcoT14 I digest); lane 1–4, MDA products from surface seawater (Droplet No. 1–4), lane 5–9, MDA products from deep seawater (Droplet No. 5–9).

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