Table 1 Taxonomic assignment of SAGs based on 16S rRNA sequences.

From: Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Droplet No.

SAG

Origin

Accession number

Taxonomy

Sequence identity to the closest relatives*

2

A

Surface seawater

LC075346

Planctomycetaceae bacterium D2

96% (AB355061)

4

B

Surface seawater

LC075347

OM182 bacterium D4

96% (AY386343)

5

C

Deep seawater

LC075348

Colwellia sp. D5

99% (JN175346)

7

D

Deep seawater

LC075349

Colwellia sp. D7

99% (JN175346)

8

E

Deep seawater

LC075350

Colwelliaceae bacterium D8

96% (HQ203946)

9

F

Deep seawater

LC075351

Flavobacteriaceae bacterium D9

98% (EU090719)

  1. The taxonomic assignment of SAGs was performed using SILVA51. The bacterium derived from SAG_C (Colwellia sp. D5) is closely related to but different from that derived from SAG_D (Colwellia sp. D7).
  2. *Numbers in parentheses correspond to the GenBank accession numbers.
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