Figure 1: Experimental paradigm to investigate the effects of sludge exposure on the fetal ovary limited to 80 day periods during early, mid or late gestation.
From: The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals
Pregnant ewes were exposed to sewage-sludge fertilized (T) pastures from: days 0 to 140 (0–140T, continuous exposure), days 0 to 80 (0–80T, early exposure), days 30 to 110 (30–110T, mid exposure) and days 60 to 140 (60–140T, late exposure). Control group pregnant ewes (C) were maintained on pastures fertilized with inorganic fertilizer from days 0 to 140 of gestation (0–140C).
