Figure 4: Analysis of differentially expressed fetal ovarian proteins following maternal exposure to sludge during critical developmental stages.
From: The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals
(a) Representative 2-D gel showing significantly altered proteins excised for identification by LC-MS/MS (Table S5). (b) Cluster analysis of altered proteins highlights the separation between the 0–140C, 0–140T and 0–80T groups from the 30–110T and 60–140T groups. Sludge exposure generally increased protein expression across all treatments, particularly in the 30–110T and 60–140T groups (c). Representative zoom-boxes and quantification are shown for (d) methylenetetrahydrofolate dehydrogenase 1, (e) serotransferrin and (f) Lamin A/C plus M/C. Each treatment group is shown with the protein circled in blue. Group means indicated by horizontal green lines. The range of control (0–140C) animals is highlighted by a blue bar and those of differing (P < 0.05) treatment groups with an orange bar and a red asterisk.
