Figure 5: Quantification and localisation of nuclear laminin proteins.
From: The fetal ovary exhibits temporal sensitivity to a ‘real-life’ mixture of environmental chemicals
(a) cropped Western blot for 75, 65 kDa LAMIN A/C and below the blue line, beta-actin load control. The full length images are shown in Supplementary Fig. 3 and gels were run under the same experimental conditions. (b) Expression (band volume) of 65 kDa LAMIN C increases in sludge-exposed fetal ovaries. The range of control (0–140C) animals is highlighted by a blue bar and those of significantly differing treatment groups with an orange bar (P < 0.05) and a red asterisk. (c) Immunoreactivity for LAMIN A/C is noticeable in nearly every cell but staining is more condensed in pre-granulosa or granulosa cells than in the oocyte or stromal cells.
