Figure 6
From: Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells
Meflin function was distinct from other members of the LIG family or proteins.
(A) A phylogenetic tree showing the evolution of representative members of the LIG family of proteins. The scale bar indicates the rate of amino acid substitutions per site. The interacting proteins for each member of the LIG family, many of which are RTKs, are also shown. (B) Interaction of Meflin with PDGFRα (left panel) and EGFR (right panel). 293FT cells were transfected with the indicated plasmids, followed by immunoprecipitation (IP) and Western blot analysis. (C) No apparent effect of Meflin-depletion in PDGF signaling in dermal fibroblasts. Lysates from control and Meflin-depleted cells stimulated with recombinant rat PDGF-BB for indicated times were subjected to Western blot analysis using the indicated antibodies. (D) Meflin regulated nuclear localization of the FoxO1 transcription factor. Western blots were used to examine whole lysates (left panel) and nuclear fractions (right panel) isolated from C3H10T1/2 cells transduced with retroviruses expressing luciferase and Meflin shRNAs. (E) C3H10T1/2 cells were transduced with retroviruses expressing GFP (control) and Meflin, followed by Western blot analysis. Overexpression of Meflin suppressed the nuclear localization of FoxO1, without apparently affecting its phosphorylation. Histone-H3 is a marker for nuclear proteins.
