Figure 2: The suppression of NOS2 expression and subsequent NO production in DENV-ADE infected K562 cells. | Scientific Reports

Figure 2: The suppression of NOS2 expression and subsequent NO production in DENV-ADE infected K562 cells.

From: Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy

Figure 2

(a) K562 cells were infected with W/WT DENV or DENV-antibody complex at a MOG of 1000, and at the indicated time points, cellular NO production was quantified by the Griess reaction. N = 3; error bars show the means ± SEM. The results from each group were compared using Student’s t test. **P < 0.01; *P < 0.05. (b,d) The expression of NOS2 at the indicated time points was determined at the gene transcription level by qRT-PCR (b) and at the protein level by western blotting (d). N = 3; error bars show the means ± SEM. (c) K562 cells were pretreated with different concentrations of SMT 1 h before DENV-3 or DENV-ADE infection, and cellular DENV genome RNA was extracted 48 h post-infection and quantified using qRT-PCR, with GAPDH as an internal control. N = 3; error bars show the means ± SEM.

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