Figure 4
The effects of amino acid composition on exponential growth of wild type and relaxed BW25113 E. coli strains.
Individual cultures in M9 medium were started from a single colony, grown up to OD600 of 0.8, diluted to OD600 of 0.1 and grown to 0.5. After that cells were gently pelleted, washed with M9 and resuspended in fresh M9 medium supplemented with either 19 amino acids (A,B) or with one amino acid (C,D). The results are shown as mean values of biological replicates (n = 2). The error bars (too small to be seen for some of the points) indicate standard error of the mean and for the sake of clarity are omitted on traces lacking specific effects.
